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List of supplements

Journal: Nature protocols

Article Title: DERIVATION OF ENTERIC NEURON LINEAGES FROM HUMAN PLURIPOTENT STEM CELLS

doi: 10.1038/s41596-019-0141-y

Figure Lengend Snippet: List of supplements

Article Snippet: DMEM/F-12, no glutamine (Life Technologies, 21331020) Essential 8™ Flex Medium Kit (Life Technologies, A2858501) Essential 6™ Medium (Life Technologies, A1516401) Neurobasal™ Medium (Life Technologies, 21103049) N-2 Supplement (CTS™, A1370701) B-27™ Supplement, serum free (Life Technologies, 17504044) MEM Nonessential Amino Acids (Corning, 25–025-CI) Glutagro ™ (Corning, 25–015-CI) BSA, Bovine Serum Albumin (Sigma, A4503) PBS, Phosphate-Buffered Saline, Ca2+- and Mg2+-free (Life Technologies, 10010023) EDTA (Corning, MT-46034CI) Accutase ™ (Stemcell Technologies, 07920) STEM-CELLBANKER® DMSO Free (Amsbio, 11897F) • BMP-4, Recombinant Human BMP-4 Protein (R&D Systems, 314-BP) Critical: Stock aliquots should be at stored −80 °C.

Techniques: Concentration Assay

a) Protocol (days 12–15) for ENC spheroid formation. NB+N2+B27; NB/N2/B27, Neurobasal medium with N2 and B27 supplement; FGF2, Recombinant Human FGF Basic; CHIR, CHIR 99021. b) Phase contrast and SOX10::GFP reporter line GFP expression of 3D spheroids on day 14. Scale bar = 200 μm.

Journal: Nature protocols

Article Title: DERIVATION OF ENTERIC NEURON LINEAGES FROM HUMAN PLURIPOTENT STEM CELLS

doi: 10.1038/s41596-019-0141-y

Figure Lengend Snippet: a) Protocol (days 12–15) for ENC spheroid formation. NB+N2+B27; NB/N2/B27, Neurobasal medium with N2 and B27 supplement; FGF2, Recombinant Human FGF Basic; CHIR, CHIR 99021. b) Phase contrast and SOX10::GFP reporter line GFP expression of 3D spheroids on day 14. Scale bar = 200 μm.

Article Snippet: DMEM/F-12, no glutamine (Life Technologies, 21331020) Essential 8™ Flex Medium Kit (Life Technologies, A2858501) Essential 6™ Medium (Life Technologies, A1516401) Neurobasal™ Medium (Life Technologies, 21103049) N-2 Supplement (CTS™, A1370701) B-27™ Supplement, serum free (Life Technologies, 17504044) MEM Nonessential Amino Acids (Corning, 25–025-CI) Glutagro ™ (Corning, 25–015-CI) BSA, Bovine Serum Albumin (Sigma, A4503) PBS, Phosphate-Buffered Saline, Ca2+- and Mg2+-free (Life Technologies, 10010023) EDTA (Corning, MT-46034CI) Accutase ™ (Stemcell Technologies, 07920) STEM-CELLBANKER® DMSO Free (Amsbio, 11897F) • BMP-4, Recombinant Human BMP-4 Protein (R&D Systems, 314-BP) Critical: Stock aliquots should be at stored −80 °C.

Techniques: Recombinant, Expressing